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第一部分 奋斗历程1

程京的中国芯——程京院士自传3

程京院士小传9

第二部分 学术贡献11

一、程京院士的学术成就与学术思想13

二、程京院士的主要学术论文16

The Extraction and Classification of Dyes from Cotton Fibres Using Different Solvent Systems16

Glycerol-enhanced Separation of DNA Fragments in Entangled Solution Capillary Electrophoresis23

Polymerase Chain Reaction Heteroduplex Polymorphism Analysis by Entangled Solution Capillary Electrophoresis30

Chip PCR.Ⅰ.Surface Passivation of Microfabricated Silicon-glass Chips、for PCR38

Chip PCR.Ⅱ.Investigation of Different PCR Amplification Systems in Microfabricated Silicon-glass Chips44

Diagnosis of Duchenne/Becker Muscular Dystrophy and Quantitative Identification of Carrier Status by Use of Entangled Solution Capillary Electrophoresis52

The Use of Capillary Electrophoresis for Point-mutation Screening61

Sample Preparation in Microstructured Devices74

Integrated Cell Isolation and Polymerase Chain Reaction Analysis Using Silicon Microfiltrer Chambers85

Degenerate Oligonucleotide Primed-polymerase Chain Reaction and Capillary Electrophoretic Analysis of Human DNA on Microchip-based Devices91

Isolation of Cultured Cervical Carcinoma Cells Mixed with Peripheral Blood Cells on a Bioelectronic Chip98

Preparation and Hybridization Analysis of DNA/RNA from E.coli on Microfabricated Bioelectronic Chips105

Response of Gene Expression in Saccharomyces cerevisiae to Amphoteriein B and Nystatin Measured by Microarrays115

Single Nucleotide Polymorphism Discrimination Assisted by Improved Base Stacking Hybridization Using Oligonucleotide Microarrays127

Preparation of Steroid Antibodies and Parallel Detection of Multianabolic Steroid Abuse with Conjugated Hapten Microarray134

Negative Dielectrophoretic Force Assisted Construction of Ordered Neuronal Networks on Cell Positioning Bioelectronic Chips142

The Design and Application of DNA Chips for Early Detection of SARS-CoV from Clinical Samples157

Parallel Profiling of Active Transcription Factors Using an Oligonucleotide Array-based Transcription Factor Assay （OATFA）167

Genomic Analysis of Anti-hepatitis B Virus（HBV） Activity by Small Interfering RNA and Lamivudine in Stable HBV-producing Cells175

The MicroArray Quality Control （MAQC） Project Shows Inter- and Intraplatform Reproducibility of Gene Expression Measurements191

Protein Chips for High-throughput Doping Screening in Athletes207

Multiplex Asymmetric PCR-based Oligonucleotide Microarray for Detection of Drug Resistance Genes Containing Single Mutations in Enterobacteriaceae211

Use of a DNA Microarray for Simultaneous Detection of Antibiotic Resistance Genes among Staphylococcal Clinical Isolates222

Construction of a Multiplex Allele-specific PCR-based Universal Array （ASPUA） and its Application to Hearing Loss Screening234

Distinctive MicroRNA Profiles Relating to Patient Survival in Esophageal Squamous Cell Carcinoma246

Analysis of the Sensitivity and Frequency Characteristics of Coplanar Electrical Cell-substrate Impedance Sensors256

Novel High-throughput Profiling of Human Transcription Factors and its Use for Systematic Pathway Mapping266

Broad Profiling of DNA-binding Transcription Factor Activities Improves Regulatory Network Construction in Adult Mouse Tissues281

Quantitative Fluorescence Correction Incorporating Forster Resonance Energy Transfer and its Use for Measurement of Hybridization Efficiency on Microarrays295

Real-time，Label-free Monitoring of the Cell Cycle with a Cellular Impedance Sensing Chip303

Integration of Sperm Motility and Chemotaxis Screening with a Microchannel-based Device312

The MicroArray Quality Control（MAQC）Ⅱ Study of Common Practices for the Development and Validation of Microarray-based Predictive Models321

Integration of Single Oocyte Trapping，in vitro Fertilization and Embryo Culture in a Microwell-structured Microfluidic Device343

MicroRNAs-372/373 Promote the Expression of Hepatitis B Virus Through the Targeting of Nuclear Factor I/B352

Validation of a Mobile Phone-assisted Microarray Decoding Platform for Signal-enhanced Mutation Detection366

Biochips for Translational Medicine375

A Microfluidic Device with Passive Air-bubble Valves for Real-time Measurement of Dose-dependent Drug Cytotoxicity through Impedance Sensing377

MicroRNA-133b is a Key Promoter of Cervical Carcinoma Development through the Activation of the ERK and AKT1 Pathways383

Use of Cellular Electrical Impedance Sensing to Assess in vitro Cytotoxicity of Anticancer Drugs in a Human Kidney Cell Nephrotoxicity Model395

Construction of a Novel Oligonucleotide Array-based Transcription Factor Interaction Assay Platform and its Uses for Profiling STAT1 Cofactors in Mouse Fibroblast Cells405

A Rapid Automatic Processing Platform for Bead Label-assisted Microarray Analysis：Application for Genetic Hearing-loss Mutation Detection415

三、程京院士的主要学术著作425

Capillary Electrophoresis of Nucleic Acids425

Biochip Technology433

生物芯片：生命信息CPU435

生物芯片技术443

四、程京院士学术年表445

程京院士论文目录445

程京院士著作目录461

程京院士国际和国内发明专利目录464

科研成果奖目录488

荣誉奖项目录490

第三部分 大师风范493

读书期间的回顾和感言495

同一屋檐下496

学术的导师，人生的楷模499

我眼中的程老师502

成长的蜕变505

记忆中程老师的点点滴滴506

念奴娇·毕业感言508

“严谨细致”成就精彩人生——观《程京：小芯片书写大人生》有感509

一种全新基因突变检测芯片的研究及在耳聋筛查中的应用511

人源转录因子芯片的构建及在信号通路系统筑图中的应用512

研制并利用神经芯片研究神经网络活动中的时空模式513

小鼠组织特异性转录调控网络的构建514

细胞内的miRNA参与调控乙型肝炎病毒表达的研究515

构建通用磁珠解码芯片平台并应用于遗传性耳聋检测516

MiR-133b在宫颈癌发生与发展中的作用研究517

精子健康评价与体外受精芯片系统的开发与应用518

用于精子筛选及体外受精的微流控芯片的构建及应用519

用于药物开发与评价的细胞芯片平台的构建520

用于精子健康评价与筛选的微流控芯片系统的构建与应用521

第四部分 社会影响523

写在生物芯片上的人生——记北京博奥生物芯片公司总裁程京博士525

美国《时代》：中国能成为创新中心吗？530

生物科技的“中国芯”532

程京：活跃的中国生物芯片领域535

程京：中国生物芯片探路者539

让生物芯片见证中国“加速度”543

程京“小”芯片 书写“大”人生551

人类健康的侦察兵557

管理创新要跟上559

程京：“芯”人问政560

什么拖了“中国创造”后腿563

程京：被“续挂”的党外代表人士566

健康产业发展的历史与未来——访中国工程院院士程京568

程京：用心缔造“中国芯”572

中国工程院院士程京：中国梦根植于健康梦577

部分新闻宣传报道列表579

第五部分 人生风采585

成长历程588

海外深造589

归国创业591

教书育人593

学术交流595

实至名归598

社会责任600