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中国科学院博士学位研究生学位论文 蛋白质“嵌入”磷脂单分子膜的结构与动态学2025|PDF|Epub|mobi|kindle电子书版本百度云盘下载
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- 赵江 著
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- ISBN:
- 出版时间:未知
- 标注页数:107页
- 文件大小:14MB
- 文件页数:114页
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图书目录
Index1
Abstract1
1.Introduction 4
1.1 General Introduction4
1.2 Fundamentals of Physical Chemistry7
1.2.1 Surface Tension and Surface Pressure7
1.2.2 Phase Transition and State of Monolayers8
1.3 Surface Properties of Lipids and Proteins10
2.Theory13
2.1 Penetration Thermodynamics13
2.1.1 Analysis Based on Gibbs Equation14
2.1.2 Analysis Based on Pethica s Equation16
2.1.3 Analysis Based on Butler s Equation20
2.2 Equation of State for Langmuir Monolayers of Amphiphilic MoleculesCapable of 2D Aggregation22
2.3 Equations of State and Adsorption Isotherms for Proteins27
2.4 Penetration Thermodynamics for Soluble Homologues29
2.4.1 Generalised Szyszkowski-Langmuir Equation30
2.4.2 Generalised Volmer Equation31
2.5 Thermodynamics of Protein Penetration into Phospholipid Monolayers35
2.6 Penetration Dynamics38
3.Materials and Methods41
3.1 Substances41
3.1.1 Protein41
3.1.2 Phospholipid42
3.1.3 Dodecyl Dimethyl Phosphine Oxide(C12DMPO)42
3.2 Setup43
3.2.1 Wilhelmy Plate Method43
3.2.2 Circular Trough44
3.2.3 Brewster Angle Microscopy(BAM)46
3.2.4 PM-IRRAS47
3.2.5 Grazing Incidence X-ray Diffraction(GIXD)49
4.Experimental Results51
4.1 Brewster Angle Microscopy(BAM)Experiments51
4.1.1 Pure DPPC51
4.1.2 Pure Protein53
4.1.3 3-Lactoglobulin Penetration into DPPC Monolayers55
4.1.3.1 Penetration into Monolayers in the Liquid-Expanded or55
Gaseous State55
4.1.3.2 Penetration into Monolayers in the Two Phase Coexistence62
Region62
4.1.3.3 Penetration into Monolayers in the Condensed State63
4.1.4.1 Penetration into Monolayers in the Liquid-Expanded State64
4.1.4 β-Casein Penetration into DPPC Monolayers64
4.1.4.2 Penetration into Monolayers in the Two Phase Coexistence67
Region67
4.1.4.3 Penetration into Monolayers in the Condensed State68
4.2 PM-IRRAS Experiments72
4.3 Thermodynamic Properties of DPPC-Protein Mixed Layer78
4.4 GIXD Experiments80
4.5 Penetration of Dodecyl Dimethyl Phosphine Oxide(C12DMPO)into DPPCMonolayers83
5.Discussion90
6.Conclusion95
Bibliography97
Publication104
Acknowledgement105
Curriculum Vitae107
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