现代组织化学技术和方法 英文2025|PDF|Epub|mobi|kindle电子书版本百度云盘下载

现代组织化学技术和方法 英文PDF格式电子书版下载

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Chapter 1 Introduction1

Section 1 The contents and theories in Histochemistry1

Section 2 The basic requirements of Histochemistry methods4

Chapter 2 The Tissue Preparation6

Section 1 Tissue collection6

Section 2 Fixation6

Section 3 Tissue washing-up,dehydration and clearance15

Section 4 Embedding17

Section 5 Sectioning18

Section 6 Adherence and mounting20

Section 7 Buffer22

Chapter 3 The Carbohydrate and its Derivatives Histochemistry24

Section 1 Classification24

Section 2 Histochemistry methods26

Chapter 4 The Nucleic Acid Histochemistry33

Section 1 DNA Histochemical demonstration33

Section 2 Comparison demonstration of DNA and RNA Histochemistry35

Chapter 5 The Lipid Histochemistry39

Section 1 Overview39

Section 2 Demonstration of lipids with physical methods40

Section 3 Demonstration of lipids with chemical methods42

Chapter 6 Enzyme Histochemistry44

Section 1 Enzyme and its basic histochemical theory44

Section 2 Histochemistry for common enzymes49

Chapter 7 The Basic Theory of Immunohistochemistry54

Section 1 Basic Immunology54

Section 2 Common markers and their detection57

Section 3 Basic conditions63

Chapter 8 Common Methods Used in Immunohistochemistry74

Section 1 Principles74

Section 2 Immunofluorescence method76

Section 3 Immunoenzyme technique80

Section 4 Avidin-biotin method83

Section 5 Protein A method87

Section 6 Immunogold method and immuno gold-silver method89

Chapter 9 Specificity and Sensitivity of Immunohistochemistry92

Section 1 Specificity and specific staining92

Section 2 Control experiment93

Section 3 The methods to enhance immunohistochemical sensitivity95

Chapter 10 The Double-Staining in Immunohistochemistry100

Section 1 Double-staining Immunohistochemistry on serial section100

Section 2 Immunofluorescence double labeling technique100

Section 3 Immunoenzyme double staining technique102

Section 4 Immunoenzyme-Immunofluorescence double staining109

Section 5 Immunoenzyme-Immunogold double staining109

Chapter 11 The Lectin Histochemistry111

Section 1 Characteristics and application of lectin111

Section 2 Application of lectin Histochemistry113

Chapter 12 The Progress of in Situ Display119

Section 1 The envision method119

Section 2 The catalyzed signal amplification method119

Section 3 The ferric oxide alternative method for HRP120

Chapter 13 In Situ Hybridization Immunohistochemistry121

Section 1 Basic principle121

Section 2 Probe preparation121

Section 3 Procedure of in situ hybridization histochemistry123

Section 4 Factors affecting in situ hybridization138

Section 5 Control test139

Chapter 14 In Situ Polymerase Chain Reaction Technique143

Section 1 Basic principle143

Section 2 Basic types143

Section 3 Procedure144

Section 4 Application of in situ PCR technology147

Chapter 15 Electron Microscopic Cytochemical Technique149

Section 1 Electron microscopic enzyme cytochemistry technology149

Section 2 Electron microscopic Immunocytochemical technique161

Chapter 16 The Quantitative Assay of Histochemistry Results177

Section 1 The photomicrography177

Section 2 The image analyzer178

Section 3 The flow cytometry180

Section 4 The laser scanning confocal microscopy185

References190

Appendix Ⅰ Preparation of Commonly Used Buffer191

Appendix Ⅱ Histochemistry and Immunohistochemistry Experiments194

Appendix Ⅲ Figures and pictures223