图书介绍

人类染色体原理与技术2025|PDF|Epub|mobi|kindle电子书版本百度云盘下载

Ram S.Verma，Arvind Babu 著
出版社：世界图书出版西安公司
ISBN：7506222272
出版时间：2000
标注页数：419页
文件大小：86MB
文件页数：433页
主题词：

PDF下载

点此进入-本书在线PDF格式电子书下载【推荐-云解压-方便快捷】直接下载PDF格式图书。移动端-PC端通用
种子下载[BT下载速度快]温馨提示：（请使用BT下载软件FDM进行下载）软件下载地址页直链下载[便捷但速度慢] [在线试读本书] [在线获取解压码]

点击复制MD5值：9b28510940c396346d2a1f3138bab96e

下载说明

人类染色体原理与技术PDF格式电子书版下载

下载的文件为RAR压缩包。需要使用解压软件进行解压得到PDF格式图书。

点击复制85GB完整离线版磁力链接到迅雷FDM等BT下载工具进行下载详情点击-查看共享计划

建议使用BT下载工具Free Download Manager进行下载,简称FDM(免费,没有广告,支持多平台）。本站资源全部打包为BT种子。所以需要使用专业的BT下载软件进行下载。如BitComet qBittorrent uTorrent等BT下载工具。迅雷目前由于本站不是热门资源。不推荐使用！后期资源热门了。安装了迅雷也可以迅雷进行下载！

（文件页数要大于标注页数，上中下等多册电子书除外）

注意：本站所有压缩包均有解压码： 点击下载压缩包解压工具

图书目录

1．INTRODUCTION1

2．TISSUE CULTURE TECHNIQUES AND CHROMOSOME PREPARATION6

Basics of Tissue Culture6

Blood8

Bone Marrow14

Prenatal Diagnosis16

Skin and Other Tissues27

Solid Tumors29

Preparation and Manipulation of Somatic Cell Hybnds31

Cell Cultures:Maintenance and Storage42

Meiotic Techniques48

Human Sperm Chromosome Karyotypes56

Bibliography63

3．BANDING TECHNIQUES72

Differential Staining Techniques72

Selective Staining Techniques77

Fluorochromes and Counterstaining Techniques90

Staining Techniques Using Antibodies109

Restriction Endonuclease/Giemsa Banding113

Bibliography127

4．SPECIALIZED TECHNIQUES134

High-Resolution Banding134

Chromosomal Fragile Sites137

Sister Chromatid Differentiation143

Replication Pattems151

Lateral Asymmetry156

Chromatin Undercondensation Techniques158

X-and Y-Chromatin164

Bibliography166

5．CHROMOSOME ANALYSIS BY FLOW CYTOMETRY172

Instrumentation175

Chromosome Preparation177

Flow Sorting Chromosomes for Library Construction179

Generation of Chromosome Paints180

Bibliography182

6．FLUORESCENCE IN SITU HYBRIDIZATION TECHNIQUES184

Metaphase Chromosomes,Interphase Nuclei,and Extended Chromatin Fibers184

Comparative Genomic Hybridization191

Multiple-Colored Chromosomes by Fluorescence In Situ Hybridization210

Multicolor Primed In Situ Labeling218

Fluorescence In Situ Hybridization in Human Sperm223

Bibliography227

7．IMMUNOCYTOGENETIC TECHNIQUES232

Antibodies Against DNA or Base Analogs Incorporated Into DNA232

Antibodies Against Chromosomal Proteins240

Immunocytogenetics and In Situ Hybridization257

Immunophenotyping of Mitotic Cells260

Bibliography264

8．MICRODISSECTION AND DIRECT PCR AMPLIFICATION OF HUMAN CHROMOSOMES271

Technical Considerations271

Microdissection Protocols275

Bibliography278

9．IN SITU HYBRIDIZATION280

Slide Preparation281

Slide Pretreatment282

Probe Preparation282

Hybridization285

Chromosomal DNA Denaturation286

Posthybridization Washing286

Hybrid Detection287

G-Banding with Wright Stain288

Appendix：Stock Solutions289

Bibliography289

10．SOUTHERN AND NORTHERN BLOTTING TECHNIQUES:PRINCIPLES AND APPLICATIONS292

Southern Blotting292

Northern Blotting298

Preparation of Radioactively Labeled Nucleic Acid Probe301

Hybridization of Filter or Membrane with a Nucleic Acid Probe303

Detection of Signals306

Applications of Blotting Techniques307

Bibliography309

Appendix：Buffers and Solutions for Protocols311

11．THE POLYMERASE CHAIN REACTION:APPLICATIONS IN GENOMIC ANALYSIS312

Historical Perspective312

Scope312

The Polymerase Chain Reaction:Principles and Basic Enzymology313

Setting Up PCR in the Laboratory:Basic Laboratory Organization319

PCR Reactions:Basic Methods321

Applications of PCR Technology to Chromosome Analysis330

Bibliography337

12．KARYOTYPE INTERPRErATION345

Historical Account345

Classification of Unbanded Chromosomes345

Nomenclature of the Banded Mitotic Chromosomes346

High-Resolution Bands of Chromosomes348

Cancer Cytogenetics349

Chromosomal Aberrations355

Evaluation of Banding Pattems358

Nomenclature for Human Meiotic Chromosomes358

Bibliography360

13．CHROMOSOMES IN CLINICAL MEDICINE362

Indications for Referral362

Classical Chromosomal Syndromes363

Cytogenetics of Spontaneous Abortion363

Chromosomal Anomalies in Prenatal Diagnosis363

Chromosome Anomalies in Mental Retardation363

Chromosomes of Human Neoplasia364

Chromosomal Basis of Oncogenesis370

Probing the Human Genome373

Bibliography406

Index411